8PAT
Structure of the E.coli DNA polymerase sliding clamp with a covalently bound peptide 3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.840, 66.570, 80.980 |
| Unit cell angles | 90.00, 129.03, 90.00 |
Refinement procedure
| Resolution | 54.810 - 1.450 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.19530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.810 | 1.450 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Number of reflections | 79966 | 3999 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 91.5 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | 0.2 M Potassium nitrate 20% PEG 3350, pH 6.8 |
