8PAQ

Structure of the small subunit of the laccase-like Nlac protein from Pleurotus eryngii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979257
Spacegroup name	P 41 21 2
Unit cell lengths	124.720, 124.720, 55.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.160 - 1.600
R-factor	0.1949
Rwork	0.194
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.780
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	58835	9324
<I/σ(I)>	14.29	0.69
Completeness [%]	99.9	99.3
Redundancy	26.2
CC(1/2)	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.1 M Imidazole at pH 8.0 and 2.5 M NaCl