8PAG
Crystal structure of the ectodomain of Norway rat pestivirus E2 glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I23 |
Synchrotron site | Diamond |
Beamline | I23 |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 2.7552 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 151.920, 151.920, 59.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.040 - 3.500 |
R-factor | 0.2697 |
Rwork | 0.269 |
R-free | 0.28270 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.168 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 3.625 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.169 | 5.688 |
Rmeas | 0.170 | 5.729 |
Rpim | 0.021 | 0.663 |
Number of reflections | 8374 | 684 |
<I/σ(I)> | 23.79 | 0.97 |
Completeness [%] | 89.8 | 75.66 |
Redundancy | 70 | 72.2 |
CC(1/2) | 1.000 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M sodium formate, 20% PEG 3350 |