8P9R

Structure of the periplasmic domain of ExbD from E. coli in complex with TonB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.3799
Spacegroup name	P 41
Unit cell lengths	49.360, 49.360, 63.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.640 - 1.520
R-factor	0.2115
Rwork	0.210
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.048
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.070	1.610
High resolution limit [Å]	1.520	1.520
Rmeas	0.061	0.816
Number of reflections	46421	7485
<I/σ(I)>	21.45	2.45
Completeness [%]	99.9	99.9
Redundancy	13.3
CC(1/2)	0.990	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	10 %w/v Glycerol, 3M ammonium sulfate