8P9D

Crystal structure of p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	40.729, 113.186, 135.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.400 - 2.700
R-factor	0.21656
Rwork	0.215
R-free	0.25164
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.080	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.083	0.848
Rmeas	0.098	1.010
Rpim	0.039	0.393
Number of reflections	17853	2555
<I/σ(I)>	11.8	2
Completeness [%]	99.5
Redundancy	6	6.2
CC(1/2)	0.998	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	20% PEG3350, 0.1M bis-tris propane pH 7.0, 0.2M salicylic, sodium salt