8P91
Hfq from Chromobacterium haemolyticum; a P6 space group monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 65.145, 65.145, 27.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.320 - 1.400 |
| R-factor | 0.169 |
| Rwork | 0.155 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.909 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.320 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.095 | 0.730 |
| Rmeas | 0.106 | 0.856 |
| Rpim | 0.045 | 0.442 |
| Total number of observations | 68788 | 2424 |
| Number of reflections | 13426 | 663 |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.1 | 3.7 |
| CC(1/2) | 0.996 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | #23 of JBScreen Nuc-Pro 1 (Jena Bioscience) 15% PEG4000, 50 mM Tris-HCl, pH 7.5, 150 mM KCl, 20 mM MgCl2 |
