8P8Q
Recombinant Ym1 crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.331, 60.009, 60.115 |
Unit cell angles | 90.00, 94.66, 90.00 |
Refinement procedure
Resolution | 42.400 - 1.792 |
R-factor | 0.1742 |
Rwork | 0.173 |
R-free | 0.19860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.910 |
Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180808) |
Data scaling software | Aimless (version 0.7.4) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.920 | 1.900 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmeas | 0.247 | 1.121 |
Number of reflections | 33407 | 5172 |
<I/σ(I)> | 5.28 | 1.54 |
Completeness [%] | 99.2 | 96 |
Redundancy | 4.06 | |
CC(1/2) | 0.979 | 0.566 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Condition A7 of the Hampton Research Crystal Screen HT (0.1 M sodium cacodylate pH 6.5, 1.4 M sodium acetate) Cryo-protection: mother liquor supplemented with 30% (v/v) glycerol |