8P5S

Crystal structure of the homohexameric 2-oxoglutarate dehydrogenase OdhA from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	H 3 2
Unit cell lengths	150.987, 150.987, 314.335
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.530 - 2.459
R-factor	0.2061
Rwork	0.204
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8p5r
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.520	2.700
High resolution limit [Å]	2.460	2.460
Rpim	0.047	0.466
Number of reflections	38261	1913
<I/σ(I)>	15.5	1.6
Completeness [%]	94.4	63.8
Redundancy	10.4
CC(1/2)	0.998	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Hepes-NaOH pH 7.5, 5% (w/v) PEG 4000, 30% (v/v) methylpentanediol (MPD)