8P3I

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1721

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	61.367, 76.601, 118.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.360 - 2.000
R-factor	0.2319
Rwork	0.229
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.360	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	38565	3800
<I/σ(I)>	3.39
Completeness [%]	88.0
Redundancy	1.9
CC(1/2)	0.997	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M NH4SO4, 0.1 M MES, pH 6.0, 15% PEG 4K