8P3G

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1704

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-31
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	62.299, 76.031, 118.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.420 - 2.130
R-factor	0.1929
Rwork	0.190
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.919
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.420	2.206
High resolution limit [Å]	2.130	2.130
Number of reflections	32270	3161
<I/σ(I)>	4.2
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.981	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M NH4SO4, 0.1 M MES, pH6.0, 15% PEG 4K