8P2Q

Crystal structure of Hen Egg White Lysozyme co-crystallized with 10 mM TbXo4-OH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.648
Spacegroup name	P 43 21 2
Unit cell lengths	77.143, 77.143, 37.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.570 - 1.690
R-factor	0.161
Rwork	0.159
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.570	1.780
High resolution limit [Å]	1.690	1.690
Rmerge	0.066	0.192
Rpim	0.025	0.054
Number of reflections	13258	1874
<I/σ(I)>	34.3	15.8
Completeness [%]	99.9	99.4
Redundancy	24.3	23.8
CC(1/2)	0.999	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	950 mM sodium chloride, 100mM sodium acetate pH=4.6. Drops were set by mixing one volume of protein solution at 20mg/ml with one volume of 10mM TbXo4 and with one volume of the well solution.