8P2N
Polymeric form of the BTB domain of ZBTB8A from Xenopus laevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 153.695, 153.695, 153.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.600 - 3.100 |
| R-factor | 0.2401 |
| Rwork | 0.238 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.374 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.029 | 0.751 |
| Number of reflections | 11832 | 2074 |
| <I/σ(I)> | 16 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 65.3 | 66.3 |
| CC(1/2) | 0.999 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | PEG200, Na/K phosphate, NaCl |
