8P1B

Lysozyme structure solved from serial crystallography data collected at 2 kHz with JUNGFRAU detector at MAXIV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-12-05
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.12716
Spacegroup name	P 43 21 2
Unit cell lengths	78.300, 78.300, 37.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.940 - 1.700
R-factor	0.1616
Rwork	0.160
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.449
Data reduction software	CrystFEL (0.10.0)
Data scaling software	CrystFEL (0.10.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	24844	2416
<I/σ(I)>	16.3	0.04
Completeness [%]	100.0	100
Redundancy	810	186
CC(1/2)	0.998	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3	293	22% NaCl, 6.4% polyethylene glycol 6000, 80 mM sodium acetate, pH 3.0