8OXL
crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 293.15 |
Detector technology | PIXEL |
Collection date | 2022-06-03 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.552, 57.552, 121.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.820 - 1.560 |
R-factor | 0.1921 |
Rwork | 0.191 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.435 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.820 | 1.650 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 29943 | 8514 |
<I/σ(I)> | 8.18 | |
Completeness [%] | 97.7 | |
Redundancy | 3.3 | |
CC(1/2) | 0.998 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 8.2 |