8OXK

crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	125.941, 28.955, 29.175
Unit cell angles	90.00, 96.67, 90.00

Refinement procedure

Resolution	31.270 - 1.370
R-factor	0.1691
Rwork	0.168
R-free	0.18590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.640
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.270	1.470
High resolution limit [Å]	1.370	1.380
Number of reflections	21484	1177
<I/σ(I)>	12.25
Completeness [%]	79.3
Redundancy	6.9
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.16 M Calcium acetate hydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 14.4% w/v Polyethylene glycol 8,000