8OXH

crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 65 2 2
Unit cell lengths	62.451, 62.451, 202.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.190 - 2.500
R-factor	0.266
Rwork	0.263
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.132
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4933)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.200	2.650
High resolution limit [Å]	2.500	2.500
Number of reflections	8696	1350
<I/σ(I)>	24.36
Completeness [%]	99.9
Redundancy	18.3
CC(1/2)	1.000	0.402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20 % w/v Polyethylene glycol 3 350, 200 mM Sodium fluoride