8OWV

H6 and F2 nanobodies bound to SARS-CoV-2 spike RBD

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-10
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	57.775, 59.438, 145.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.438 - 1.730
Rwork	0.197
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.403
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.520	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.105
Rpim	0.031
Number of reflections	53452	2634
<I/σ(I)>	11.5
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000