8OWI

Crystal structure of the corona-targeting domain of CENP-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 42 21 2
Unit cell lengths	84.018, 84.018, 65.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.420 - 2.140
R-factor	0.2176
Rwork	0.216
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.574
Data scaling software	STARANISO
Phasing software	Arcimboldo
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.420	2.340
High resolution limit [Å]	2.140	2.140
Rpim	0.026	0.563
Number of reflections	9178	82
<I/σ(I)>	17.9	1.3
Completeness [%]	68.8	15
Redundancy	24.8
CC(1/2)	1.000	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Potassium citrate tribasic monohydrate pH 8.3, 20% w/v polyethene glycol 3,350, with 30% glycerol (cryoprotectant).