8OW4
2.75 angstrom crystal structure of human NFAT1 with bound DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979181 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 101.497, 94.426, 129.449 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 82.242 - 2.750 |
| Rwork | 0.216 |
| R-free | 0.27280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.835 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.242 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.067 | 0.506 |
| Rmeas | 0.088 | 0.665 |
| Rpim | 0.056 | 0.427 |
| Number of reflections | 30970 | 4185 |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 97.3 | 99.9 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.996 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.1 M sodium cacodylate pH 6.0, 8% w/v PEG 8000 |
