8OSI

Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-25
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 32 2 1
Unit cell lengths	151.400, 151.400, 61.969
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.950 - 2.420
R-factor	0.19756
Rwork	0.196
R-free	0.23104
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.616
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.950	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.101	1.437
Rmeas	0.104	1.483
Rpim	0.025	0.360
Total number of observations	520158	47835
Number of reflections	31109	3135
<I/σ(I)>	15.8	1.7
Completeness [%]	99.4
Redundancy	16.7	15.3
CC(1/2)	0.994	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.1 M MMT buffer, pH 8.0, 25% PEG 1500