8OS2
Structure of the human LYVE-1 (lymphatic vessel endothelial receptor-1) hyaluronan binding domain in an unliganded state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 70.810, 70.810, 75.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.770 - 1.640 |
R-factor | 0.1845 |
Rwork | 0.183 |
R-free | 0.22280 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.969 |
Data scaling software | xia2 |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.770 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmeas | 0.059 | 1.951 |
Number of reflections | 303577 | 1181 |
<I/σ(I)> | 21 | 1.2 |
Completeness [%] | 100.0 | |
Redundancy | 12.5 | |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.5M NaCl, 0.1M Sodium/Potassium Phosphate pH 5.5 |