8OS1
X-ray structure of the Peroxisomal Targeting Signal 1 (PTS1) of Trypanosoma Cruzi PEX5 in complex with the PTS1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.331, 69.936, 71.106 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 48.220 - 2.100 |
| R-factor | 0.19544 |
| Rwork | 0.193 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | AutoProcess |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35080 | 2248 |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 95.9 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.959 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M MgCl2, 0.1 M Tris pH 8.5 and 30% (w/v) PEG 4000 |
