8ORX

Structure of the murine LYVE-1 (lymphatic vessel endothelial receptor-1) hyaluronan binding domain in an unliganded state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	C 1 2 1
Unit cell lengths	31.370, 58.320, 52.350
Unit cell angles	90.00, 94.62, 90.00

Refinement procedure

Resolution	29.160 - 1.540
R-factor	0.1706
Rwork	0.169
R-free	0.19470
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.857
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.21rc1_4899)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.160	1.640
High resolution limit [Å]	1.540	1.540
Rmeas	0.067
Number of reflections	18090	2315
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	6.5
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG3350 0.1M bis-Tris. Protein concentration 17.6 mg/ml.