8ORP

Crystal structure of Drosophila melanogaster alpha-amylase in complex with the inhibitor acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 1 21 1
Unit cell lengths	80.080, 72.540, 98.210
Unit cell angles	90.00, 98.41, 90.00

Refinement procedure

Resolution	29.570 - 2.000
R-factor	0.176
Rwork	0.174
R-free	0.21160
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	75240	10118
<I/σ(I)>	9
Completeness [%]	99.8	99.7
Redundancy	3.7
CC(1/2)	0.993	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 % (v/v) PEG monomethyl ether 550, 0.1 M sodium chloride, 0.1 M bicine buffer (pH 9.0) and 10 mM strontium chloride. Soak in a solution containing 10 mM acarbose.