8OQD

Dirhodium tetraacetate/ribonuclease A adduct in the P3221 space group (1 h soaking)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-08-24
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5406
Spacegroup name	P 32 2 1
Unit cell lengths	67.142, 67.142, 64.791
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.637 - 1.540
Rwork	0.195
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	3.198
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.980	1.560
High resolution limit [Å]	1.540	1.540
Rmerge	0.077	2.388
Rpim	0.041	1.763
Number of reflections	25583	1242
<I/σ(I)>	12.3	0.4
Completeness [%]	100.0	99.8
Redundancy	4.1	2.6
CC(1/2)	0.998	0.087

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	2.5 M NaCl 3.3 M HCOONa 0.1 M Acetate buffer