8OQ0

Crystal structure of tailspike depolymerase (APK09_gp48) from Acinetobacter phage APK09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.7749
Spacegroup name	H 3 2
Unit cell lengths	88.688, 88.688, 421.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.150 - 2.590
R-factor	0.19312
Rwork	0.189
R-free	0.27157
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.379
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.150	2.700
High resolution limit [Å]	2.590	2.590
Rmerge	0.339	2.467
Rmeas	0.349	2.566
Rpim	0.081	0.674
Total number of observations	377770	31386
Number of reflections	20409	2302
<I/σ(I)>	10.9	1.7
Completeness [%]	99.1
Redundancy	18.5	13.6
CC(1/2)	0.993	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	2% PEG200, 100mM HEPES pH 7.5, 20% tacsimate pH 7.0