8OPI

VHH Z70 mutant 1 in interaction with PHF6 Tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 65 2 2
Unit cell lengths	135.165, 135.165, 35.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.240 - 1.830
Rwork	0.198
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.827
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.240	44.240	1.870
High resolution limit [Å]	1.830	8.970	1.830
Rmerge	0.155	0.045	3.123
Rmeas	0.159	0.046	3.200
Rpim	0.034	0.010	0.700
Number of reflections	17371	17371	191
<I/σ(I)>	20
Completeness [%]	100.0
Redundancy	39.5	30.3	39.3
CC(1/2)	1.000	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	1.7M Ammonium Sulfate, 4.25% Isopropanol, 15% lLycerol