8OP0

VHH Z70 mutant 20 in interaction with PHF6 Tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 65 2 2
Unit cell lengths	135.309, 135.309, 35.508
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.230 - 1.540
Rwork	0.160
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.575
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.290	44.290	1.570
High resolution limit [Å]	1.540	8.430	1.540
Rmerge	0.071	0.057	1.788
Rmeas	0.072	0.058	1.830
Rpim	0.015	0.013	0.385
Number of reflections	28067	191	1055
<I/σ(I)>	31.5
Completeness [%]	97.5
Redundancy	40.8	31.2	42.8
CC(1/2)	0.999	0.995	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.095M tri-Sodium citrate pH 5.6, 19% isopropanol, 15% Glycerol