8ONX
High resolution structure of Chaetomium thermophilum MAP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 1021-09-04 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.9737 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.938, 73.806, 82.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.020 - 1.300 |
R-factor | 0.1619 |
Rwork | 0.161 |
R-free | 0.17680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.954 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.530 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rpim | 0.015 | 0.288 |
Number of reflections | 90066 | 4362 |
<I/σ(I)> | 23 | |
Completeness [%] | 99.9 | |
Redundancy | 12.3 | |
CC(1/2) | 0.999 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350 |