8ONW
Crystal structure of the hetero-dimeric complex from Archaeoglobus fulgidus PRC1 and PRC2 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.086, 102.633, 112.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.020 - 2.290 |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.036 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.020 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmeas | 0.048 | |
| Number of reflections | 30556 | 463 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 89.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.500 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% MPD, 40 mM magnesium chloride, 50 mM sodium cacodylate pH 6 |
