8OLW
Structure of Oceanobacillus iheyensis group II intron before the first step of splicing in the presence of K+, Ca2+ and intronistat B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.147, 94.133, 222.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 4.000 |
| R-factor | 0.22225 |
| Rwork | 0.220 |
| R-free | 0.25804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.097 | |
| Number of reflections | 16065 | 1442 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 97.8 | |
| Redundancy | 4.6 | |
| CC(1/2) | 1.000 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM potassium acetate, 100 mM potassium chloride, 100 mM calcium chloride, 50 mM HEPES sodium, pH 7.0, 4.5% PEG8000 |
