8OKH

Crystal structure of Bdellovibrio bacteriovorus Bd1399

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	90.159, 90.159, 112.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.470 - 2.170
R-factor	0.1887
Rwork	0.186
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.367	2.433
High resolution limit [Å]	2.170	2.170
Number of reflections	16227	812
<I/σ(I)>	12.8
Completeness [%]	64.8
Redundancy	13.1
CC(1/2)	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1M Sodium formate; 0.1M Ammonium acetate; 0.1M Sodium citrate tribasic dihydrate; 0.1M Potassium sodium tartrate tetrahydrate; 0.1M Sodium oxamate, 100 mM Tris (base); 100 mM BICINE pH 8.5, 20 % v/v Ethylene glycol, 10% w/v PEG 8000