8OK3

Structure of the C-terminal domain of the Bdellovibrio bacteriovorus Bd2133 fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	68.760, 68.760, 68.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.750 - 1.500
R-factor	0.1508
Rwork	0.150
R-free	0.15920
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	Aimless
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.750	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	17441	1266
<I/σ(I)>	10.3
Completeness [%]	99.1	98
Redundancy	3.4	3.4
CC(1/2)	0.996	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.03M magnesium chloride, 0.03M calcium chloride, 0.1 M imidazole/MES pH 6.5, 12.5 % v/v MPD, 12.5 % PEG 1000, 12.5 % w/v PEG 3350