8OJP

Human galectin 1 in complex with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	53.350, 61.960, 100.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.775 - 1.710
Rwork	0.168
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.928
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.780	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.041	0.672
Number of reflections	36768	2200
<I/σ(I)>	20.3
Completeness [%]	99.8
Redundancy	5.4
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8, 28% PEG3350, 0.1 M Li2SO4, 1% beta-mercapthoethanol