8OJ3

Crystal structure of the Candida albicans 80S ribosome in complex with geneticin G418 (rotated state)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	300.290, 294.060, 442.550
Unit cell angles	90.00, 99.77, 90.00

Refinement procedure

Resolution	266.820 - 3.500
R-factor	0.2489
Rwork	0.249
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19rc4-4035)
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	266.820	266.820	3.550
High resolution limit [Å]	3.500	50.000	3.500
Rmerge	0.516	0.051	3.239
Rmeas	0.534	0.053	3.356
Rpim	0.139
Number of reflections	929602	271	38940
<I/σ(I)>	4.86
Completeness [%]	97.9
Redundancy	14.6
CC(1/2)	0.997	0.998	0.090

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside