8OIB
Trichomonas vaginalis riboside hydrolase in complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-22 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.034, 156.998, 88.729 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 88.725 - 2.440 |
| Rwork | 0.189 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.730 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rpim | 0.058 | 0.317 |
| Number of reflections | 50817 | 2518 |
| <I/σ(I)> | 6.1 | 2.1 |
| Completeness [%] | 98.0 | 99.2 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.993 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.85 | 291.15 | 200 mM ammonium citrate dibasic pH 4.85, 15% w/v PEG 5000 monomethyl ether, 17.5% (v/v) glycerol |
