8OIA

Trichomonas vaginalis riboside hydrolase in complex with D-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	C 1 2 1
Unit cell lengths	116.995, 135.622, 94.075
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	43.981 - 2.300
Rwork	0.234
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.379
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.981	2.250
High resolution limit [Å]	2.200	2.200
Rpim	0.136	0.425
Number of reflections	71792	4593
<I/σ(I)>	5.2	1.2
Completeness [%]	99.9	100
Redundancy	5.9	5.8
CC(1/2)	0.981	0.425

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.85	291.15	200 mM ammonium citrate dibasic pH 4.85, 15% PEG 3300