8OI4
Metagenomic Beta-galactosidase from Glycoside Hydrolase family GH154
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-19 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.316, 94.581, 108.264 |
Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
Resolution | 47.400 - 1.760 |
R-factor | 0.15325 |
Rwork | 0.152 |
R-free | 0.17110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.264 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 177698 | 5125 |
<I/σ(I)> | 6.1 | 0.8 |
Completeness [%] | 96.0 | 56.1 |
Redundancy | 2.53 | |
CC(1/2) | 0.987 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16% (w/v) PEG 3350, 100 mM HEPES-NaOH, pH 7.5, 150 mM NaCl |