8OI2
Crystal structure of Alb1 megabody in complex with human serum albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 370.359, 73.819, 66.130 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 33.860 - 3.300 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.609 |
| Data reduction software | AutoProcess |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.270 | 3.530 |
| High resolution limit [Å] | 3.250 | 3.300 |
| Rmerge | 0.044 | 0.937 |
| Rpim | 0.044 | 0.932 |
| Number of reflections | 43671 | 4847 |
| <I/σ(I)> | 11 | 1.1 |
| Completeness [%] | 98.4 | 99.6 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.998 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.03M Sodium fluoride, 0.03M sodium bromide, 0.03M sodium iodide, 0.1M Tris BICINE pH 8.5, 12% v/v ethylene glycol, 6% w/v PEG 8000 |
