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8OH8

Crystal structure of the cysteine-rich Gallus gallus urate oxidase in complex with the 8-azaxanthine inhibitor under reducing conditions (space group P 21 21 21)

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]100
Detector technologyPIXEL
Collection date2021-07-20
DetectorDECTRIS PILATUS3 6M
Wavelength(s)0.97950
Spacegroup nameP 21 21 21
Unit cell lengths102.220, 124.400, 125.770
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution66.880 - 2.120
Rwork0.185
R-free0.21410
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle1.451
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]66.8802.160
High resolution limit [Å]2.1202.120
Rmerge0.1862.540
Rpim0.0700.934
Number of reflections915054515
<I/σ(I)>8.10.9
Completeness [%]100.0100
Redundancy88.2
CC(1/2)0.9970.363
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5291.15His6-tagged GgUOX at approx. 22 mg/ml in 50 mM Tris-HCl pH 8.0, 10 mM NaCl, 1 mM TCEP-HCl was incubated with a 20-fold molar excess of AZA. Crystals were obtained by mixing the protein with 0.8M K/Na tartrate tetrahydrate, 0.1 M HEPES pH 7.5

229380

PDB entries from 2024-12-25

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