8OH8
Crystal structure of the cysteine-rich Gallus gallus urate oxidase in complex with the 8-azaxanthine inhibitor under reducing conditions (space group P 21 21 21)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.220, 124.400, 125.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.880 - 2.120 |
Rwork | 0.185 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.451 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.880 | 2.160 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.186 | 2.540 |
Rpim | 0.070 | 0.934 |
Number of reflections | 91505 | 4515 |
<I/σ(I)> | 8.1 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8 | 8.2 |
CC(1/2) | 0.997 | 0.363 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | His6-tagged GgUOX at approx. 22 mg/ml in 50 mM Tris-HCl pH 8.0, 10 mM NaCl, 1 mM TCEP-HCl was incubated with a 20-fold molar excess of AZA. Crystals were obtained by mixing the protein with 0.8M K/Na tartrate tetrahydrate, 0.1 M HEPES pH 7.5 |