8OGA

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-24
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.581, 81.581, 234.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.191 - 2.200
Rwork	0.185
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.988
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.960	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	24491	2058
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	29.5
CC(1/2)	0.995	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Tris pH 7.5, 1.66M Lithium acetate