8OG9

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	82.981, 82.981, 237.602
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.277 - 2.945
Rwork	0.191
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.277	3.120
High resolution limit [Å]	2.940	2.940
Number of reflections	11003	1680
<I/σ(I)>	7.2
Completeness [%]	99.6
Redundancy	18.5
CC(1/2)	0.994	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES pH6.5, 2.13M Lithium acetate