8OFV

Human adenovirus type 53 fiber-knob protein complexed with sialic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91188
Spacegroup name	P 1 2 1
Unit cell lengths	93.555, 60.406, 243.686
Unit cell angles	90.00, 99.88, 90.00

Refinement procedure

Resolution	92.170 - 1.770
R-factor	0.20937
Rwork	0.208
R-free	0.22826
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.537
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.170	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.168	1.707
Rmeas	0.195	1.967
Rpim	0.097	0.966
Number of reflections	258349	12686
<I/σ(I)>	6	0.6
Completeness [%]	98.7	97.7
Redundancy	3.8	3.9
CC(1/2)	0.685	0.327

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M PCTP buffer, 25 % w/v PEG 1500