8OFV
Human adenovirus type 53 fiber-knob protein complexed with sialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91188 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 93.555, 60.406, 243.686 |
| Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
| Resolution | 92.170 - 1.770 |
| R-factor | 0.20937 |
| Rwork | 0.208 |
| R-free | 0.22826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.537 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.170 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.168 | 1.707 |
| Rmeas | 0.195 | 1.967 |
| Rpim | 0.097 | 0.966 |
| Number of reflections | 258349 | 12686 |
| <I/σ(I)> | 6 | 0.6 |
| Completeness [%] | 98.7 | 97.7 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.685 | 0.327 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 0.1 M PCTP buffer, 25 % w/v PEG 1500 |
