8OFM
Structure of the ALDEHYDE DEHYDROGENASE 5F1 (ALDH5F1) from the moss Physcomitrium patens in complex with NAD in an extended conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-03 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 212.309, 212.309, 187.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.630 - 1.831 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.19600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | FFT |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.200 | 2.000 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.195 | 2.207 |
| Rpim | 0.021 | |
| Number of reflections | 103740 | 5187 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 73.4 | |
| Redundancy | 20 | |
| CC(1/2) | 0.998 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | lithium sulfate, sodium succinate |
