8KHW

The crystal structure of glycosaminoglycan lyase GAGase VII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	98.407, 98.407, 134.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.400
R-factor	0.2122
Rwork	0.207
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.870	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.098	0.720
Rmeas	0.100	0.737
Rpim	0.020	0.158
Number of reflections	49305	2737
<I/σ(I)>	20.6	4.3
Completeness [%]	99.7	99.85
Redundancy	23.6	21.4
CC(1/2)	0.999	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES (pH 7.0), 36% (v/v) PEG 400