8KHN
Crystal structure of human methionine aminopeptidase 12 (MAP12) in complex with two cobalt ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97957 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.916, 80.978, 49.033 |
Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
Resolution | 27.350 - 1.510 |
R-factor | 0.1936 |
Rwork | 0.193 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.350 | 27.350 | 1.600 |
High resolution limit [Å] | 1.510 | 4.480 | 1.510 |
Rmerge | 0.091 | 0.035 | 0.922 |
Rmeas | 0.107 | 0.042 | 1.071 |
Number of reflections | 46575 | 1826 | 7348 |
<I/σ(I)> | 8.61 | ||
Completeness [%] | 98.8 | ||
Redundancy | 3.7 | ||
CC(1/2) | 0.997 | 0.997 | 0.679 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 0.1M HEPES pH 7.2, 0.1M Carboxylic acids, 17% (v/v) MPD, 17% (w/v) PEG 1000, 17% (w/v) PEG 3350 |