8KHM

Crystal structure of human methionine aminopeptidase 12 (MAP12) in the unbound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97957
Spacegroup name	C 1 2 1
Unit cell lengths	77.398, 81.204, 48.893
Unit cell angles	90.00, 102.33, 90.00

Refinement procedure

Resolution	27.670 - 1.390
R-factor	0.1899
Rwork	0.189
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.670	27.670	1.480
High resolution limit [Å]	1.390	4.140	1.390
Rmerge	0.062	0.022	0.881
Rmeas	0.073	0.025	1.023
Number of reflections	58324	2292	9301
<I/σ(I)>	12.83
Completeness [%]	99.3
Redundancy	3.8
CC(1/2)	0.999	0.999	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.1M HEPES pH 7.2, 0.1M Carboxylic acids, 17% (v/v) MPD, 17% (w/v) PEG 1000, 17% (w/v) PEG 3350