8KGS

Structure of African swine fever virus topoisomerase II in complex with dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-10-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	85.077, 85.077, 210.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.780 - 2.603
R-factor	0.2023
Rwork	0.200
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.203	1.019
Rpim		0.339
Number of reflections	14604	708
<I/σ(I)>	11	1.38
Completeness [%]	100.0	99.3
Redundancy	19	9
CC(1/2)	0.996	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	21% PEG 3350, 100 mM Hepes (pH 7.2) and 200 mM MgCl2