8KG4
Crystal Structure of M- and C-Domains of the shaft pilin LrpA from Ligilactobacillus ruminis - orthorhombic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98030 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.825, 50.853, 125.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.710 - 1.200 |
R-factor | 0.16956 |
Rwork | 0.169 |
R-free | 0.18761 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.657 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.710 | 1.223 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.096 | 0.779 |
Rmeas | 0.102 | 0.834 |
Rpim | 0.034 | 0.292 |
Number of reflections | 84143 | 4207 |
<I/σ(I)> | 12.8 | 4.6 |
Completeness [%] | 99.6 | 94.6 |
Redundancy | 8.6 | 7.7 |
CC(1/2) | 0.996 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1 M sodium acetate pH 4.6, 25% PEG 4000 |