8KEU

Crystal Structures of Endo-beta-1,4-xylanase II mutant T2CT28C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.119, 59.298, 69.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.060 - 1.100
R-factor	0.136
Rwork	0.135
R-free	0.14930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.022
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.060	1.139
High resolution limit [Å]	1.100	1.100
Number of reflections	78472	6874
<I/σ(I)>	23.857	2
Completeness [%]	98.1	87.11
Redundancy	11.7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	20% PEG 8000, 0.2M NaI, 0.1M MES